Short-period microtremors are measured at about 80 sites along a line crossing the Tokyo Bay area. The purpose of this study is to extract a reliable parameter from microtremors for site characterization in which source effects are excluded. The period of peak horizontal-to-vertical amplitude ratio of microtremors is found to be in good agreement with the natural site period, irrespective of source function of the site. It is shown that the good agreement is attributed to the predominance of Rayleigh waves in microtremor ground motions.